Journal:

Article Title: Feline Non-repetitive Mitochondrial DNA Control Region Database for Forensic Evidence

doi: 10.1016/j.fsigen.2010.01.013

Figure Lengend Snippet: Mitotype Distribution Among Random Bred Cats

Article Snippet: Fancy Cat Breed Population Genetics With respect to breeds, 22 of 26 breeds had mitotype A. Mitotypes A – C accounted for 59.7% of all types.

Techniques:

Journal:

Article Title: Feline Non-repetitive Mitochondrial DNA Control Region Database for Forensic Evidence

doi: 10.1016/j.fsigen.2010.01.013

Figure Lengend Snippet: Mitotype Distribution Among Fancy Breed Cats

Article Snippet: Fancy Cat Breed Population Genetics With respect to breeds, 22 of 26 breeds had mitotype A. Mitotypes A – C accounted for 59.7% of all types.

Techniques:

Journal:

Article Title: Feline Non-repetitive Mitochondrial DNA Control Region Database for Forensic Evidence

doi: 10.1016/j.fsigen.2010.01.013

Figure Lengend Snippet: Table of Universal Mitotype Defining Nucleotides Mitotype defining sites are provided based upon the first published mt sequence [ 10 ] and the Sylvester Reference Sequence [ 26 ]. For the SRS, both the sequence position and occupying nucleotide are indicated. OL indicates outlier groups 1,2 and 3.

Article Snippet: Fancy Cat Breed Population Genetics With respect to breeds, 22 of 26 breeds had mitotype A. Mitotypes A – C accounted for 59.7% of all types.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9 R239X Mice

doi: 10.1371/journal.pone.0158344

Figure Lengend Snippet: Coq9 mRNA levels in MEFs ( A ) and mHPCs ( B ). COQ9 protein levels in MEFs ( C ) and mHPCs ( D ). Q: 1 hit in MEFs, 200 μl, non-concentrated; R: 1 hit in MEFs, 100 μl, 10x-concentrated; S: 1 hit in MEFs, 25 μl, 10x-concentrated; T: 1 hit in MEFs, 5 μl, 10x-concentrated; U: 1 hit in HSCs, 400 μl, 23.5x-concentrated; V: 2 hits in HSCs 400 μl, 23.5x-concentrated; 1: 7–9 days after transduction in HSCs; 2: 12–16 days after transduction in HSCs. Data are expressed as mean ± SD. *** P < 0.001, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; +++ P < 0.001, Coq9 R239X-CCoq9WP cells versus Coq9 R239X cells; (one-way ANOVA with a Tukey's post hoc test; n = 4–6 for each group). #: a non-specific band is detected in Coq9 R239X cells with the anti-COQ9 antibody.

Article Snippet: Amplification was performed with quantitative real-time PCR, by standard curve method, with specific Taqman probes (from Applied Biosystems) for the targeted gene mouse Coq9 (Mm00804236_m1) and the mouse Hprt probe as a standard loading control (Mm01545399_m1).

Techniques: Transduction

Journal: PLoS ONE

Article Title: Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9 R239X Mice

doi: 10.1371/journal.pone.0158344

Figure Lengend Snippet: Levels of COQ7 in MEFs ( A ) and mHSCs ( B ). R: 1 hit in MEFs, 100 μl, 10x-concentrated; U: 1 hit in HPCs, 400 μl, 23.5x-concentrated; V: 2 hits in HPCs 400 μl, 23.5x-concentrated; 1: 7–9 days after transduction in HSCs; 2: 12–16 days after transduction in HSCs. Data are expressed as mean ± SD. ** P < 0.01, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; *** P < 0.001, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; + P < 0.05, Coq9 R239X-CCoq9WP cells versus Coq9 R239X cells; +++ P < 0.001, Coq9 R239X-CCoq9WP cells versus Coq9 R239X cells; (one-way ANOVA with a Tukey's post hoc test; n = 4–6 for each group).

Article Snippet: Amplification was performed with quantitative real-time PCR, by standard curve method, with specific Taqman probes (from Applied Biosystems) for the targeted gene mouse Coq9 (Mm00804236_m1) and the mouse Hprt probe as a standard loading control (Mm01545399_m1).

Techniques: Transduction

Journal: PLoS ONE

Article Title: Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9 R239X Mice

doi: 10.1371/journal.pone.0158344

Figure Lengend Snippet: Levels of CoQ 9 ( A ) and DMQ 9 /CoQ 9 ratio ( B ). Representative chromatographs of the three different groups ( C-E ). Q: 1 hit in MEFs, 200 μl, non-concentrated; R: 1 hit in MEFs, 100 μl, 10x-concentrated; S: 1 hit in MEFs, 25 μl, 10x-concentrated; T: 1 hit in MEFs, 5 μl, 10x-concentrated. Data are expressed as mean ± SD. * P < 0.05, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; *** P < 0.001, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; +++ P < 0.001, Coq9 R239X-CCoq9WP cells versus Coq9 R239X cells; (one-way ANOVA with a Tukey's post hoc test; n = 4–6 for each group).

Article Snippet: Amplification was performed with quantitative real-time PCR, by standard curve method, with specific Taqman probes (from Applied Biosystems) for the targeted gene mouse Coq9 (Mm00804236_m1) and the mouse Hprt probe as a standard loading control (Mm01545399_m1).

Techniques:

Journal: PLoS ONE

Article Title: Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9 R239X Mice

doi: 10.1371/journal.pone.0158344

Figure Lengend Snippet: Levels of CoQ 9 ( A ) and DMQ 9 /CoQ 9 ratio ( B ). Representative chromatographs of the three different groups ( C-E ). U: 1 hit in HSCs, 400 μl, 23.5x-concentrated; V: 2 hits in HPCs 400 μl, 23.5x-concentrated; 1: 7–9 days after transduction in HPCs; 2: 12–16 days after transduction in HSCs. Data are expressed as mean ± SD. * P < 0.05, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; ** P < 0.01, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; *** P < 0.001, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; +++ P < 0.001, Coq9 R239X-CCoq9WP cells versus Coq9 R239X cells; (one-way ANOVA with a Tukey's post hoc test; n = 4–6 for each group).

Article Snippet: Amplification was performed with quantitative real-time PCR, by standard curve method, with specific Taqman probes (from Applied Biosystems) for the targeted gene mouse Coq9 (Mm00804236_m1) and the mouse Hprt probe as a standard loading control (Mm01545399_m1).

Techniques: Transduction

Journal: PLoS ONE

Article Title: Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9 R239X Mice

doi: 10.1371/journal.pone.0158344

Figure Lengend Snippet: Oxygen consumption rate (OCR) profile (A), spare respiratory capacity (B) and cell index (C) in MEFs. Vertical arrows indicate the time of addition of oligomycin (olyg), FCCP and rotenone/antymicin (Rot/Ant). 5×10 4 cells were plated in each well. Data are expressed as mean ± SD. * P < 0.05, Coq9 R239X and Coq9 R239X-CCoq9WP cells versus Coq9 +/+ cells; ++ P < 0.01, Coq9 R239X-CCoq9WP cells versus Coq9 R239X cells; (one-way ANOVA with a Tukey's post hoc test; n = 4–6 for each group).

Article Snippet: Amplification was performed with quantitative real-time PCR, by standard curve method, with specific Taqman probes (from Applied Biosystems) for the targeted gene mouse Coq9 (Mm00804236_m1) and the mouse Hprt probe as a standard loading control (Mm01545399_m1).

Techniques: